中文摘要：

p33^ING1作为一种重要的抑瘤基因，在乳腺癌及胃癌等恶性肿瘤细胞中的mRNA表达水平显著低于癌旁正常组织细胞中的表达，并导致这些肿瘤细胞的侵袭转移能力增强，对化疗药物及γ射线和紫外线处理的敏感性降低．然而，促进该基因转录水平升高能直接抑制肿瘤细胞的生长和阻止细胞的恶性转化，因而诱导该基因mRNA表达升高的方法被广泛认为是治疗乳腺癌等恶性肿瘤的新手段．

英文摘要：

In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells, p33^ING1 mRNA level in cellular total RNA was detected quantitatively based on MB assay. During the operation, the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33^ING1 were measured in vitro. The results were as follows： p33^ING1 mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time. The MTr results also proved that high expression of p33^ING1 mRNA can increase cellg sensitivity to chemical drug 5-FU. The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.