中文摘要：

随着纳米技术的迅速发展，以纳米颗粒（NPs）作为基因／药物载体的体外靶向治疗引起了广泛的关注，并取得了一定的成效．但在这些研究过程中都会遇到如何提高细胞对载体颗粒有效吞噬的难题，因此，在开发基于NPs作为基因／药物载体的同时，与细胞吞噬NPs作用相关的基础研究也引起了广泛重视．硅纳米颗粒（SiNPs）因其具有生物相容性好等优点在细胞水平上得到了一定的应用，但有关细胞吞噬SiNPs的研究并不多见．

英文摘要：

The uptake of silica nanoparticles（SiNPs） with positive surface charge and negative surface charge by HepG cells, MCF-7 cells and L-02 cells were investigated by using the RITC doped in SiNPs as a synchronous fluorescent signal indicator. The experimental results show that the uptake of SiNPs by the three kinds of cells were all a concentration-, charge-, time-, and serum-dependent endocytic process. To the same kind of SiNPs, the uptake by the three kinds of cells all enhanced with increasing of the SiNPs concentration and incubation time and gradually reached saturation. However, the effect of serum was different to the uptake of SiNPs with different surface charge. The results display that serum existed in culture medium decreased the cellular uptake of NSiNPs. While the serum existed in culture medium would not affect the cellular uptake of PSiNPs. These results provide a theoretical foundation for the further modification of SiNPs, which could improve the stability of SiNPs in the medium and made it be well applied to biomedicine.