中文摘要：

由猪小肠中纯化鉴定Daintain／AIF-1，并以其为免疫原免疫BALB／c小鼠，用杂交瘤技术制备得到4株稳定分泌抗体的单克隆细胞株。对单抗效价、亚类、亲和常数进行分析，4株单抗都属于IgG1（k）。又经Westemblot证实，所制备的抗体能特异性与Daintain／AIF-1结合。

英文摘要：

Daintain/AIF-1 is a recently discovered cytokine-like polypeptide. This bioactive peptide is involved in transplant rejection, autoimmune diseases and tumor generation and progress etc. Daintain/AIF-1 from porcine intestine was isolated and identified and used as antigen to manufacture monoclonal antibodies （Mabs）. Using hybridization technique, four cell clones producing Mabs against Daintain/AIF-1 were obtained. Isotypes, titer,affinity of the Mabs were also analysed. Those Mabs belong to G1 subclass with k light chain. The specificity of the Mabs was further verified by Western blot. In first time the monoclonal antibodies against Daintain/AIF-1 was prepared and identified, which will be useful tools for further investigation of transplant rejection,autoimmune diseases and cancer diagnosis.