中文摘要：

从黑寡妇蜘蛛的毒液的 alpha-Latrotoxin 通过细胞外的 Ca (2+) 流入和细胞的信号 transduction 小径导致并且扩充 neurotransmitter 和荷尔蒙版本。由使用记录的整个房间电流和电容，将关入笼中的 Ca (2+) 的光分解作用，和 Ca (2+) 微萤光计和 amperometry，我们在老鼠在 exocytosis 上调查了 alpha-latrotoxin 的刺激效果和机制胰腺的贝它房间， LbetaT2 房间和 latrophilin plasmid-transfected INS-1 房间。我们的数据显示了那：(1 ) alpha-latrotoxin 通过允许阳离子的毛孔和随后的 Ca (2+) 的形成增加了 cytosolic Ca (2+) 集中有细胞外的 Ca (2+) 的存在的流入；(2 ) alpha-latrotoxin 在正常洗澡答案刺激了 exocytosis，它分泌物上的刺激效果在 Ca (2+) 被根除免费洗澡答案；并且(3 ) alpha-latrotoxin 通过蛋白质 kinase C 的激活敏化熔化的分子的机械并且由紫外光的闪光增加了房间的反应到 Ca (2+) photolyzed。在摘要， alpha-latrotoxin 通过 Ca (2+) 流入导致了 exocytosis 并且由熔化机械的促进感受性加速了泡熔化。

英文摘要：

α-Latrotoxin from the venom of black widow spider induces and augments neurotransmitter and hormone release by way of extracellular Ca^2＋ influx and cellular signal transduction pathways. By using whole cell current and capacitance recording, the photolysis of caged Ca^2＋, and Ca^2＋ microfluorometry and amperometry, we investigated the stimulating effect and mechanism of ot-latrotoxin on exocytosis in rat pancreatic β cells, LβT2 cells and latrophilin plasmid-transfected INS-1 cells. Our data indicated that： （1） α- latrotoxin increased cytosolic Ca^2＋ concentration through the formation of cation-permitting pores and subsequent Ca^2＋ influx with the presence of extracellular Ca^2＋; （2） α-latrotoxin stimulated exocytosis in normal bath solution and its stimulating effect on secretion was eradicated in Ca^2＋-free bath solution; and （3） α- latrotoxin sensitized the molecular machinery of fusion through activation of protein kinase C and increased the response of cells to Ca^2＋ photolysed by a flash of ultraviolet light. In summary, α-latrotoxin induced exocytosis by way of Ca^2＋ influx and accelerated vesicle fusion by the sensitization of fusion machinery.