中文摘要：

目的探讨体外培养条件下，EGF对舌背黏膜上皮细胞周期和凋亡的影响。方法DispaseⅡ和胰蛋白酶联合分离舌背黏膜上皮细胞，以含浓度1、5、10μg／LEGF RPMI-1640的作用后16、32和48h，流式细胞术检测细胞周期和凋亡。结果舌背黏膜上皮细胞在体外培养条件下，EGF作用小鼠舌背黏膜上皮细胞32和48h后，表现为抗凋亡作用，在48h显示浓度依赖性；EGF作用兔舌背黏膜上皮细胞16h，表现抗凋亡作用但不显示浓度依赖性。结论EGF能在不同时间段调控小鼠和兔的舌背黏膜上皮细胞凋亡。

英文摘要：

Objective To investigate the effect of EGF on cell cycle and apoptosis in epithelia of dorsal lingual mucosa from mice and rabbits in vitro. Methods Dispase Ⅱ and trypsin were combined to digest the lingual dorsum epidermis, and the epithelia were obtained from healthy mice and rabbits. After the lingual dorsal epithelia were incubated in 10% RPMI-1640 withl, 5, 10 μg/L EGF （control group 0 μg/L） by 16 h, 32 h, 48 h, flow cytometric analysis was used to analyze the cell cycle distribution and apoptosis. Results Compared with the control group, cell cycle distribution and apoptosis showed distinct after the period of time with EGF. EGF could inhibit the apoptosis of the epithelia from mice at 32 h and 48 h, as well as inhibit the apoptosis of the epithelia from rabbits at 16 h. In addition, the effect of anti-apoptosis showed dose-dependent in the epithelia from mice at 48 h. Conclusion EGF could inhibit the apoptosis of the lingual dorsum epithelial cells from mice and rabbit by altering the cell cycle distribution.