中文摘要：

目的：探讨千金苇茎汤辅助治疗舌鳞癌的分子机制。方法：体外培养SAS和TCA-8113舌鳞癌细胞,撤除血清模拟体内抗血管生成药物治疗环境,千金苇茎汤3个部位作用撤血清舌鳞癌细胞72 h,MTT法检测细胞增殖活性,流式细胞术检测细胞周期分布和凋亡,ELISA检测细胞培养上清前列腺素E2（PGE2）含量。结果：与撤血清对照相比,千金苇茎汤07部位能够降低撤血清舌鳞癌细胞的增殖活性（生长抑制率max34.36%）,促进SAS和TCA-8113细胞凋亡率升高（P〈0.05）,抑制细胞分泌PGE2（P〈0.05）,且具有剂量和时间依赖性。结论：千金苇茎汤07部位促进撤血清舌鳞癌细胞凋亡的机制与抑制PGE2合成相关,为深入研究千金苇茎汤辅助治疗恶性肿瘤提供了新的思路和方法。

英文摘要：

Objective： To investigate the mechanism of Qianjin Weijing prescription（QJWJ） on promoting apoptosis of the human tongue squamous cell carcinoma SAS and TCA-8113 with serum withdrawal.Method： The SAS and TCA-8113 were treated with serum withdrawal to imitate the condition caused by the chemical therapy of anti-angiogenesis.The assistant effects on anti-tumor of three parts extracted from QJWJ were investigated by SAS and TCA-8113 with serum withdrawal in vitro.MTT assay was used to evaluate the activities of cell proliferation,and flow cytometric analysis was conducted to analyze the cell cycle distribution and apoptosis.The level of the prostaglandin E 2（PGE 2） in the cell culture supernatant was detected by enzyme-linked immunosorbent assay（ELISA）.Result： In comparison with the control,the results showed the 07 part of QJWJ could distinctly promote the inhibitory effects on the activities of cell proliferation in SAS and TCA-8113 cells.The maximum growth inhibition rate 34.36%,and the apoptosis of human squamous cell carcinoma（P 0.05） by flow cytometric analysis.The level of the PGE 2 in the cell culture supernatant was inhibited by 07 part of the QJWJ by ELISA assay（P 0.05）.Conclusion： The results suggested that the promoted effects of QJWJ on apoptosis of the hunman tongue squamous cell carcinoma with serum withdrawal was mediated by the inhibition of PGE 2 releasing.The research provided a new way to explore the scientific interests of QJWJ in adjuvant treatment of malignant cancers.