中文摘要：

目的：研究替米沙坦对醋酸去氧皮质酮（DOCA）高血压大鼠肾脏保护作用和足细胞Podocalyxin蛋白表达的影响。方法：18只雄性Wistar大鼠随机分为对照组（A组）、高血压组（B组）和替米沙坦治疗组（C组）。单肾切除后皮下注射DOCA（5 mg/只,2次/周）和饮用盐水（1%Nacl＋0.2%KCl）建立高血压模型。C组用替米沙坦[5mg/（kg.d）]灌胃。测定第4、8周尿蛋白。8周后处死动物,测血肌酐（SCr）、血钠、血钾,取肾组织PAS染色观察病理变化,免疫组化法检测肾脏podocalyxin表达变化。结果：B组4周始血压和尿蛋白较A组逐渐升高（P〈0.05）。B组肾小球硬化指数和间质纤维化指数均明显高于A组（P〈0.05）,podocalyxin蛋白表达较A组降低。C组血压、尿蛋白和病理损伤较B组减轻,podocalyxin表达显著高于B组（P〈0.05）。结论：替米沙坦通过上调足细胞podocalyxin表达,减轻DOCA高血压大鼠肾损伤。

英文摘要：

Objective： To investigate the effect of telmisartan on the renal function and podocalyxin ex- pression in podoeytes of deoxycorticosterone acetate （DOCA） hypertensive Wistar rats. Methods： Eighteen male Wistar rats were randomly assigned into three groups： control group, hypertensive group, and telmisartan-treated group （n= 6 respectively）. Blood pressure and proteinuria were measured after 4 and 8 weeks. Renal pathological changes were quantified by the indices of glo- merulosclerosis （GS） and interstitial fibrosis （IF）. Expression of podocalyxin was analyzed by immunohistochemical staining. Results： The proteinuria and hypertension in hypertensive rats were significantly alleviated after telmisart~ n treatment. In addition, down-regulation of podoca- lyxin expression in glomeruli and pathological damages in hypertensive rats were prevented by telmisartan intervention. Conclusion： Telmisartan exerts a protective effect on improving expres- sion of podocalyxin in glomeruli to prevent the progression of hypertensive renal damage.