中文摘要：

目的：研究表皮生长因子受体（epidermal growth factor receptor,EGFR）抑制剂AG1478对人肝细胞癌细胞HuH7黏附、迁移及侵袭的影响及其相关细胞内信号转导通路,探讨EGFR在肿瘤转移中的作用。方法：用AG1478处理人肝细胞癌细胞HuH7;用MTT法检测细胞的黏附能力变化;用Transwell小室观察体外细胞迁移能力变化;Transwell小室结合Matrix胶检测肿瘤细胞侵袭能力的变化;用Western blot法检测Erk2及Paxillin蛋白表达。结果：AG1478（20μmol/L）处理后,细胞的黏附、迁移和侵袭能力分别下降了52%、49%和70%（P〈0.01）,Erk2及Paxillin的表达水平也明显降低（P〈0.01）。结论：EGFR与人肝细胞癌细胞HuH7的黏附、迁移和侵袭性密切相关,并可能通过Erk2及Paxillin信号转导通路调控人肝细胞癌的发展。

英文摘要：

Objective：To investigate the effect of epidermal growth factor receptor （EGFR） inhibitor AG1478 on adhesion, migration and invasion of HuH7 cell line and its molecular mechanism. Methods：HuH7 cells were treated with AG1478. Cell adhesion assay and Matrix-gel were used to examine the capability of adhesion,migration and invasion. Western blotting was employed to detect the expression of Erk2 and Paxillin in HuH7 cells. Results： AG1478 （20 μmol/L） significantly inhibited the adhesion of HuH7 cells by 52%. And Matrix-gel experiment showed that AG1478（20 μmol/L） decreased the migration and invasion ability of HuH7 cells in vitro by 49% and 70%,respectively（P 〈 0.01 ）. After the treatment of AG1478 for 24 h,the expression of Erk2 and Paxillin were decreased remarkably （P 〈 0.01 ）. Conclusion： Our findings suggested that EGFR signal transduction pathway might play a key role on the capability of adhesion,migration and invasion in human HCC cell line HuH7,which could be partly related to the mechanism of Erk2 and Paxillin signal pathway.