中文摘要：

目的：探讨PGE2对人肝癌细胞HuH7VEGF表达的影响及其可能涉及的信号转导通路。方法：用PGE2、EP1-4四种受体激动剂、SQ22536＋PGE2、H89＋PGE2处理HuH7细胞,Real-time PCR、Western blot、ELISA等检测VEGFmRNA、蛋白表达的变化。结果：10μmol/L PGE2处理HuH7细胞24h后,VEGFmRNA的表达水平上升了264%（P〈0.01）、细胞培养上清中VEGF蛋白表达水平上升了24%（P〈0.01）;10μmol/L PGE2处理HuH7细胞6、12、24h后,胞浆中VEGF蛋白的表达水平分别上升了13.6%、25.7%、39.6%（P〈0.01）;10μmol/L EP2受体激动剂处理HuH7细胞24h后,胞浆、细胞培养上清中VEGF蛋白表达水平与对照组相比分别上升了32.3%、20.1%（P〈0.01）,而10μmol/L EP1、EP3、EP4受体激动剂处理HuH7细胞24h后,VEGF的表达水平无明显改变;30μmol/L PKA抑制剂（H89）、500μmol/L腺苷酸环化酶抑制剂（SQ22536）处理HuH7细胞24h后,胞浆VEGF的表达水平分别为对照组的91.6%、89.6%,细胞培养上清中VEGF的表达水平分别为对照组的98.3%、91.9%,与PGE2组相比均有统计学差异（P〈0.01）。结论：PGE2可上调HuH7细胞VEGF的表达,其作用机制可能是通过EP2受体激活cAMP-PKA信号转导通路所致。

英文摘要：

Objective：To explore VEGF expression induced by PGE2 in human hepatocellular carcinoma cells HuH7 and the possible mechanisms of signal transduction pathways. Methods：HuH7 cells were treated with PGE2,EP1-4 receptor agonist,SQ22536＋PGE2, H89＋PGE2. VEGF mRNA and protein expression were examined by Real-time PCR,Western blot and ELISA in HuH7 cells. Results： The expression of VEGF mRNA in HuH7 cells were increased by 264%（P〈0.01）,and the expression of VEGF in culture supernatant of HuH7 cells were increased by 24%（P〈0.01）after treated with PGE2（10 μmol / L） for 24 h. The expression of VEGF in cytoplasm of HuH7 cells were increased by 13.6%,25.7%,39.6% （P〈0.01）after treated with PGE2 （10 μmol/L） for 6 h,12 h,24 h respectively. The expression of VEGF in cytoplasm and culture supernatant of HuH7 cells were increased by 32.3% and 20.1% （P〈0.01）after treated with EP2 receptor agonist（10 μmol / L） for 24 h respectively,but have no changes after treated with EP1,EP3,EP4 （10 μmol / L） receptor agonist. After the treatment of H89 （30 μmol / L） and SQ22536 （500 μmol / L） for 24 h,the expression of VEGF in cytoplasm of HuH7 cells was decreased by 91.6% and 89.6%,and the expression of VEGF in culture supernatant of HuH7 cells was decreased by 98.3% and 91.9%,respectively,vs PGE2（P〈0.01）. Conclusion：It is suggested that PGE2 might up-regulate the expression of VEGF through EP2 receptor of HuH7 cells,which could be partly related to the cAMP-PKA signal conduction pathway.