中文摘要：

应用AB-8大孔树脂对芦蒿秸秆总黄酮进行富集和纯化,优化得到树脂富集纯化芦蒿秸秆总黄酮的最佳工艺条件;通过显色反应、紫外特征光谱吸收对纯化后的产物进行初步鉴定。结果表明：AB-8大孔树脂具有较好的吸附和解吸性能,最佳吸附洗脱条件为：pH4.0,上样质量浓度1.476mg/mL,上样流速1mL/min,上样体积为树脂体积的2.5倍,洗脱剂为体积分数60%的乙醇,洗脱流速1mL/min,洗脱体积为1.5～2.0BV左右。在该条件下,纯化产品中总黄酮的纯度由5.80%增加到30.26%;显色反应及紫外特征吸收峰显示,芦蒿秸秆总黄酮中可能含有黄酮醇、异黄酮、黄烷酮和双氢黄酮类,并且结构中有邻位双OH存在。

英文摘要：

This study aimed at establishing the optimum conditions for purifying total flavonoids from Artemisia selengensis Turcz with AB-8 macroporous resin.In addition,we preliminarily identified the resulting purified product by means of chromogenic reaction and UV adsorption spectroscopy.AB-8 possessed excellent adsorption and desorption efficiencies for total flavonoids.The optimum adsorption and desorption conditions were determined as follows： 2.26 BV of 1.476 mg/mL sample solution at pH 4.0 was flowed through AB-8 column at a rate of 1 mL/min and then desorbed with 1.5—2.0 BV of 60% ethanol at a flow rate of 1 mL/min.The purity of the purified product obtained under these conditions was 30.26%,compared to 5.80% for the crude total flavonoids extract.Chromogenic reaction and UV characteristic adsorption indicated that total flavonoids from Artemisia selengensis Turcz might contain flavonols,isoflavones,flavonone and double hydrogen flavonols with ortho-dihydroxyl groups in their structure.