中文摘要：

以苹果属山荆子为试材,根据Nramp1同源序列保守区设计两对简并引物进行PCR,结合RACE技术获得了3＇末端和5＇末端片段,将3段片段序列拼接,根据拼接序列获取了MbNramp1基因全长cDNA序列。MbNramp1cDNA长2090bp,包含一个长度为1656bp的开放阅读框,编码551个氨基酸的多肽,分子量约为59.7kD。该基因编码的蛋白是一个膜蛋白,76%以上的氨基酸为非极性。MbNRAMP1与二价铁、锰转运蛋白NRAMP基因家族保守性很高,具有NRAMP1金属转运蛋白家族基因典型特征,即含有推断的N–端相连的糖基化位点、10个预测的跨膜结构域（TMs）,在第6和第7跨膜结构域间有一个相同的转运基序（CTM）。低铁胁迫时MbNramp1在根中表达量增加。

英文摘要：

Degenerate primers corresponding to the conserved motifs of NRAMP1 in plants were used to amplify specific DNA fragments from Malus baccata（L.）Borkh.roots cDNA.Then the gene specific primers were designed based on the obtained specific DNA fragments,the 3＇ end and 5＇ end fragments were amplified by RACE.A 2 090 bp full length cDNA MbNramp1 containing a 1 656 bp ORF was obtained based on specific DNA fragments.MbNramp1 encodes a polypeptide of 551 amino acids with a predicted molecular mass of 59.7 kD and a membrane protein with more than 76% non-polar amino acids.All conserved features of NRAMP described previously were present in the predicted MbNRAMP1 sequence,such as putative N-linked glycosylation sites,10 transmembrane domains（TMS）and a consensus transporter motif（CTM）located between Ⅵ and Ⅶ TM.Expression of MbNramp1 was increased in roots under iron deficiency,and its mRNA accumulation patterns differed with the induction time.