中文摘要：

以山葡萄品种左山一的叶片和花芽为材料，对cDNA—AFLP体系的几个关键因素进行摸索，建立了适宜山葡萄的cDNA—AFLP反应体系。结果表明，提取山葡萄的花芽RNA适宜用改良的CTAB法，提取叶片RNA适宜用SDS．酸酚法。在2×Y／T Buffer的作用下，500ng的cDNA利用Mse I与EcoR I双酶切4h即可酶切完全，将16％连接过夜的产物稀释5倍作为预扩增的模板，预扩增产物稀释20倍作为选扩模板能获得带型稳定可重复性好的结果。

英文摘要：

The cDNA-AFLP system for Vitis amurensis Rupr was established after optimizing several key factors affecting cDNA-AFLP analysis. The result indicated that CTAB method could be used for extracting ideal RNA from leaf and inflorescence of Vitis amurensis Rupr,while SDS-acidic phenol method for extracting RNA from bud;500 ng cDNA was digested completely by Eco R I and Mse I for 6 hours. The reducible results was obtained when the ligation products were diluted to 5 times for preamplification template and preamplification productsthe were diluted to 20 times for selec- tive amplification.