中文摘要：

为研究蜂房哈夫尼亚菌（Hafniaalvei）Ha-01生物被膜形成的过程及不同培养条件（碳源、pH值、NaCl质量分数、黏附材料）对其生物被膜形成的影响，采用超声波平板法及扫描电镜法研究不同培养条件下菌株Ha-01生物被膜活菌数及生长情况，并通过添加外源标准群体感应信号分子（N-酰基高丝氨酸内酯（N-acyl-homoserinelactones，AHLs））中的C6-HSL研究了AHLs与其生物被膜形成的关系。结果显示，菌株Ha-01生物被膜的形成与培养时间密切相关；在不同碳源的培养条件下形成生物被膜能力不同，其中在以木糖为培养基时形成量最大，达到7.51（lg（CFU/cm2）），与在LB培养基中相比增加10.28%；在中性培养条件下更利于其生物被膜的形成，活菌数为7.77（lg（CFU/cm2））；在NaCl质量分数为2%时，其生物被膜产生量最大，活菌数为7.18（lg（CFU/cm2））；在不同黏附材料上生物被膜形成能力从大到小依次为铝片、锌片、玻璃片，活菌数分别为7.22、6.48、6.11（lg（CFU/cm2））；且添加C6-HSL量越多，其生物被膜产生能力越强。研究表明，培养条件能够影响菌株Ha-01生物被膜形成，且AHLs可以调控其生物被膜形成。

英文摘要：

This study aimed to investigate the process of biofilm formation of Hafnia alvei Ha-01,and to examine theinfluences of different culture conditions(carbon source,pH,NaCl concentration,and adhesive material)on the number ofviable cells in biofilm formed by Hafnia alvei and its growth properties by the ultrasonic plate method and scanningelectron microscopy(SEM),and we also assessed the relationship between exogenous N-acyl-homoserine lactone(C6-HSL)as a quorum-sensing(QS)signal molecule and the biofilm formation of Hafnia alvei.The results indicatedthat the biofilm formation of strain Ha-01was closely related to incubation time.The biofilm formation ability of Ha-01varied depending on carbon source,and when xylose was used as carbon source,its biofilm formation ability reachedthe strongest level of7.51(lg(CFU/cm2)),which was increased by10.28%as compared with that in LB medium.The biofilm formation ability of Ha-01was stronger under neutral conditions,with the viable cell count reaching7.77(lg(CFU/cm2)).At a NaCl concentration of2%,the biofilm formation was the highest,reaching7.18(lg(CFU/cm2)).The biofilm formation ability of Ha-01on different material surfaces was deceased in theorder of Al,Zn,and glass.The numbers of viable cells on these materials were7.22,6.48,and6.11(lg(CFU/cm2)),respectively.The biofilm formation ability became stronger with the increase of exogenous C6-HSL.In conclusion,culture conditions could affect the biofilm formation of Hafnia alvei,which could be regulated by AHLs.