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Spatial variability of cyanobacterial community composition in Sanya Bay as determined by DGGE fingerprinting and multivariate analysis
  • ISSN号:0253-2654
  • 期刊名称:《微生物学通报》
  • 时间:0
  • 分类:Q958.15[生物学—动物学] Q938[生物学—微生物学]
  • 作者机构:[1]Key Laboratory of Marine Bio-resourses Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences Guangzhou 510301, China, [2]State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China, [3]National Experiment Station of Tropical Marine Biology, Chinese Academy of Sciences, Sanya 572000, China, [4]Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • 相关基金:supported by the National Science and Technology Supporting Program(2009BAB44B03);the National High-tech R&D Program of China(2012AA092104);the National Natural Science Foundation of China(40776069,40676091 and 41006069);the National Basic Research Program of China (2010CB833800);the Knowledge Innovation Program of Chinese Academy of Sciences(KSCX2-EW-G-12);the Sanya Station Database and the Information System of CERN
中文摘要:

在表面和 Sanya 海湾的底部水的 cyanobacterial 社区在 4 月 24 日被调查并且 25, 2010。流动 cytometry 证明在表面和底部层的全部的 cyanobacterial 丰富从 0.7 ? 匠湡慹???? 祣湡扯捡整楲污吗??

英文摘要:

The cyanobacterial communities in the surface and bottom waters of Sanya Bay were investigated on April 24 and 25, 2010. Flow cytometry showed that the total cyanobacterial abundance in the surface and bottom layers ranged from 0.?×10^4 to 2.38×10^4 cells mL^-1 and from l×10^4 to 1.8x104 cells mL^-1, respectively. Cyanobacterial diversity was analyzed using a molecular fingerprinting technique called denaturing gradient gel electrophoresis (DGGE), followed by DNA sequencing. The results were then interpreted through multivariate statistical analysis. Differences in the compositions of cyanobacterial communities were observed in the surface and bottom waters at the same station, with some bands obtained from both the surface and bottom layers, whereas some bands were present only in one layer. The predominant cyanobacterial species of the excised DGGE bands were related to Synechococcus or Synechococcus-like species (56.2%). Other phylogenetic groups identified included Chroococcidiopsis (6.3%), Cyanobium (6.3%) and some unclassified cyanobacteria (31.2%). A redundancy analysis (RDA) was conducted to reveal the relationships between the cyanobacterial community composition and environmental factors. Analysis results showed that the spatial variations in the cyanobacterial community composition in surface waters was significantly related to chlorophyll a (Chla), the biochemical oxygen demand (BOD), nitrate and phosphate (P〈0.05). Meanwhile, the spatial variations in the bottom waters was significantly affected by nitrate, nitrite, and phosphate (P〈0.05). Environmental parameters could explain 99.3% and 58.3% of the variations in the surface and bottom layers, respectively.

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期刊信息
  • 《微生物学通报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院微生物研究所 中国微生物学会
  • 主编:赫荣乔
  • 地址:北京市朝阳区北辰西路一号院3号中国科学院微生物研究所内B401室
  • 邮编:100101
  • 邮箱:xuj@sun.im.ac.cn
  • 电话:010-64807511
  • 国际标准刊号:ISSN:0253-2654
  • 国内统一刊号:ISSN:11-1996/Q
  • 邮发代号:2-817
  • 获奖情况:
  • 1992年中国科学技术协会优秀学术期刊三等奖,1992年国家科委中共中央宣传部新闻出版署“全国优...,2000年中国科学院优秀期刊三等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),波兰哥白尼索引,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:29487