欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的研究木犀草素、芹菜素与牛血清白蛋白（BSA）的相互作用机制。方法主要应用荧光猝灭法及非辐射能量转移原理。结果测得不同温度下，木犀草素、芹菜素与牛血清白蛋白的结合常数，确定两种黄酮小分子对牛血清白蛋白荧光的猝灭是静态猝灭过程，并依据能量转移原理求得其结合距离和能量转移效率。结论木犀草素、芹菜素与牛血清白蛋白间有较强的结合作用，且结合力以疏水作用力为主；B（3’）-OH，B（4’）-OH邻位对黄酮与牛血清白蛋白的结合具有增强作用。
英文摘要：
Aim To study the interaction mechanism of bovine serum albumin （BSA） with luteolin and apigenin. Methods Fluorescence quenching method and non-radioactive energy transfer theory were used. Results The binding constants at different temperature were determined and the quenching mechanism of them were suggested as static quenching. The transfer efficiency of energy and distance between BSA and luteolin or apigenin were investigated according to the mechanism of the Forster energy transference. Conclusion The interaction between them seems to be strong and the binding force were mainly hydrophobic force. B （3＇） -OH, B （4＇） -OH strengthened the interaction of flavonoids and BSA.
同期刊论文项目
同项目期刊论文
期刊信息
位置：