中文摘要：

目的了解我国南方城市中学生多种多环芳烃（PAHs）的内暴露水平。方法于2005年6月采集南方某市某中学41名男生（16～18岁）的尿样。尿液经过酶解、固相萃取（SPE）富集、净化，采用高效液相色谱．荧光检测器检测多环芳烃羟基代谢物（HO-PAHs）。结果共检出7种HO—PAHs，分别为2-羟基萘（M、x：3．62、4．27umol／mol肌酐）、2．羟基芴（M、x：1．55、1．74umol／mol肌酐）、2-羟基菲＋3-羟基菲（M、x：0．23、0．25umol／mol肌酐）、9-羟基菲（M、x：0．45、0．72umol／mol肌酐）、4．羟基菲[M、x〈检出限（LOD）、0．02umol／mol肌酐]和1-羟基芘（M、x：0．18、0．22umol／mol肌酐）。结论 1-羟基芘的含量中位数略高于已有报道的中国东北地区（0．13umol／mol肌酐）和北京地区背景人群（0．15umol／mol肌酐），但明显高于欧美及韩国等国家同龄学生（〈0．08umol／mol肌酐）。各HO-PAHs之间较差的相关性提示，多种代谢物的联合测定可更客观地评价人体PAHs内暴露水平。

英文摘要：

Objective To investigate the human internal exposure level of several polycyelic aromatic hydrocarbons （PAHs） in the middle school students in a city in south China. Methods The urine samples from 41 middle-schoolboys （16-18 years） of the city in south china were collected in June 2005. After enzymic hydrolysis, the urine samples were enriched and cleaned by solid phase extraction, and then the urinary hydroxy-PAHs were determined by using high performance liquid chromatography with fluorescence detection. Results Seven hydroxy polycyelic aromatic hydrocarbons were detected in the urinary samples. The range, median and mean concentrations of these metabolites were ： 2-hydroxy naphthalene （0.58-14.95, 3.6：2, 4.27 umol/mol ereatinine）, 2-hydroxy fluorene （0.68-6.17, 1.55, 1.74 umol/mol creatinine）, 2-hydrol phenanthrene＋3-hydroxyl phenanthrene（0.09-0.69, 0.23, 0.25 umol/mo] creatinine）, 9-hydroxy phenanthrene （0.19-4.94, 0.45, 0.72 umo]/mol ereatinine）, 4-hydroxy phenanthrene （〈LOD-0.13, 〈LOD, 0.02 umol/mol creatinine） and 1-hydroxy pyrene （0.06-0.58, 0.18, 0.22 umo]/mol creatinine）. Conclusion The median concentration of 1-hydroxy pyrene in this study was a slightly higher than those of the background population in northeast China （0.13 umol/mol creatinine） and Beijing （0.15 umol/mol ereatinine）. However, it is obviously higher than the reported data from international middle school students in Europe, American and Keroean （〈0.08 umol/mol creatinine）. The results of spearman＇s correlation matrix between different metabolites indicate that multi-determination of hydroxy-PAH will be helpful to assess the PAH exposure level of human.