比较了不同消毒剂和处理方式对"龙纹"豇豆种子的消毒效果及无菌生长的效应。结果表明:先消毒再浸种的效果较好,其中0.1%的升汞消毒8 min的效果最佳(污染率为11%,萌发率为77%)。消毒后将种子接种在附加有300 mg/L头孢噻肟钠的培养基中,能有效减少培养过程中的污染率(7%),同时不影响无菌幼苗的生长,10 d后85%无菌苗株高可达3 cm以上。
The effects of different seed disinfection methods on Longwen cultivar cowpea seeds wereinvestigated. Optimum results were achieved by surface sterilizing the seeds with 0. 1% mercurybichloride solution for 8 minutes(contamination rate 11%,germination rate 77%). The contaminationrate can be efficiently decreased by adding 300 mg/L cefotaxime sodium to the medium after surfacesterilization(7%). This method does not affect the normal growth of plants,85% of plants reached theheight of 3 cm after 10 days.