中文摘要：

为研究BMP-6在猪卵泡发育过程中的作用,采用免疫荧光和Western blot技术检测BMP-6在猪排卵前卵泡卵母细胞、颗粒细胞以及卵泡液中的表达;在猪卵母细胞体外成熟培养过程中分别添加5、25及50 ng/mL BMP-6蛋白,观察其对猪卵母细胞在体外成熟过程中的影响。结果表明,猪排卵前卵泡的卵母细胞和颗粒细胞以及卵泡液中均存在BMP-6蛋白,体外添加不同浓度的BMP-6蛋白均能明显促进猪卵母细胞体外成熟,说明BMP-6在猪卵泡发育及猪卵母细胞的成熟过程中发挥作用。

英文摘要：

To investigate the role of BMP-6 in the development of porcine follicle,the immunofluorescence assay and Western blot were used to detect the expression of BMP-6 protein in oocytes and granulosa cells of porcine preovulatory follicles. Also,the oocytes of preovulatory follicles were cultured in vitro and different concentrations of BMP-6（ 5,25 and 50 ng/mL） were added into the culture to investigate in vitro maturation of porcine oocytes. The results showed that expression of BMP-6 protein could be detected in oocytes,granular cells and follicular fluid of preovulatory follicles,and in vitro maturation of porcine oocytes was enhanced by the addition of BMP-6 protein. The results indicate that BMP-6 is involved in the development of porcine follicles and plays a role in porcine oocyte maturation.