中文摘要：

新生儿卵巢同源盒基因（newborn ovary homeobox gene,NOBOX）是母源基因的一种,在早期卵子发生中起重要作用。为了明确NOBOX基因在猪卵巢等组织中的表达情况,本研究首先采用实时荧光定量PCR方法检测了NOBOX基因在猪不同组织中的表达,然后构建了NOBOX基因的原位杂交探针,采用原位杂交技术检测NOBOX基因在猪卵巢中的表达定位情况。结果显示,NOBOX基因在猪卵巢中的表达最高,极显著高于肾脏、肝脏、乳腺、肌肉及肾上腺等组织（P〈0.01）,而在肾脏、肝脏、乳腺、肌肉和肾上腺中,NOBOX基因表达差异不显著（P〉0.05）。在猪卵巢中NOBOX基因的表达定位于卵母细胞胞质中,在颗粒细胞中未检测NOBOX基因的表达。以上结果表明,NOBOX基因仅在猪的卵母细胞胞质中高表达,预示NOBOX基因很可能在卵母细胞发生和胚胎的早期发育过程中发挥调节作用。

英文摘要：

Newborn ovary homeobox gene（NOBOX）is one of maternal genes,which is important in early oogenesis.In order to know the expression pattern of NOBOX gene in porcine tissues,Real-time fluorescence quantitative PCR was used to detected the expression of NOBOX gene in porcine tissues,and then the probe of NOBOXgene was generated and in-situ hybridization were used to detect NOBOXgene localization in porcine ovary.The results showed that NOBOXgene expression was significantly higher in ovary than in other tissues（P〈0.01）.There was no significant difference in NOBOXgene expression among kidney,liver,mammary gland,muscle and adrenal（P〈0.05）.In ovary,NOBOXgene was localized in oocyte cytoplasm specifically,but not in granulosa cell.All these results revealed that NOBOXgene was highly expressed in the cytoplasm of procine oocyte,indicating that it played an important role in porcine oocyte and early embryo development.