中文摘要：

为评价鼠伤寒沙门氏菌鞭毛蛋白（FliC）以及鸡GM-CSF（chGM-CSF）对新城疫病毒（NDV）弱毒疫苗的免疫增强效果,本实验通过PCR扩增fliC,并合成chGM-CSF编码基因,分别构建重组质粒pBrClone30-fliC和pBrClone30-GM-CSF,拯救获得重组病毒rClone30-fliC和rClone30-GM-CSF.RT-PCR和ELISA检测结果表明：fliC和chGM-CSF基因正确插入重组病毒,并获得稳定表达;重组病毒免疫7d后重组NDV HI抗体效价检测显示,实验组平均HI效价分别达到4.5 log2和4.8 log2,而rClone30组和空白组HI效价仅为3 log2和1 log2;免疫7d后攻毒,空白组SPF鸡均死亡,rClone30组有两例发病,实验组SPF鸡全部存活并且无任何临床症状.本研究结果表明,FliC和chGM-CSF作为佐剂能够有效提高弱毒疫苗的免疫效果.

英文摘要：

To evaluate the immune enhancement of flagellin （FIiC） and chicken GM-CSF （chGM-CSF） to the attenuated New castle disease （ND） vaccine, the genes of Salmonella typhimurium fliC and chGM-CSF were amplified by PCR and inserted between F and HN NDV vector to construct pBrClone30-fliC and pBrClone30-GM-CSF the recombinant viruses of rClone30-fliC and rClone30-GM-CSF were rescued by reverse-genetics technique. The RT-PCR and ELISA detections indicated that fliC and chGM-CSF genes were stably integrated in the rescued viruses and effectively expressed in cells infected by the recombinant viruses. Additionally, the HI antibody titers against NDV in experimental groups immunized rClone30-fliC and rCIone30-GM-CSF reached 4.51og2 and 4.81og2 at 7 days post vaccination, while rClone30 group and the control group were 31og2 and llog2, respectively. Furthermore, the recombinant viruses provided better protections for chickens against the challenge than that of the parental virus.