中文摘要：

利用微局域机械力刺激，快速实时观察机械力引起的细胞问钙波传递，系统地研究了BV-2小胶质细胞问钙通讯机制．结果表明，在细胞种植密度较小且彼此未接触的情况下，旁分泌途径可介导BV-2小胶质细胞间钙波传递．在细胞密度较大且相互接触的情况下，旁分泌和间隙连接两种途径可共同介导胞间钙波传递．更为有趣的是，在体外发现BV-2小胶质细胞间存在通道纳米管类似物连接，也可介导小胶质细胞问钙波传递．综上所述，小胶质细胞间钙波传递可通过旁分泌、间隙连接和通道纳米管类似物连接三种途径介导．

英文摘要：

In the present work, we applied mechanical stimulus to study the propagations of intercellular calcium waves among BV-2 microglial cells by the fast fluorescence microscopy imaging system in vitro. The results showed that calcium signaling responded to mechanical stimulus and propagated to neighboring cells in isolated BV-2 microglial cells, suggesting that the paracrine pathway mediated intercellular calcium waves. Further data indicated that gap junction was involved in mechanically-induced intercellular calcium waves when the cells had physical contact with each other. More importantly, tunneling nanotubes （TNTs）-like structure between BV-2 microglial cells was found in vitro and mediated the propagation of intercellular calcium wave. Taken together, our studies demonstrated that three distinct pathways, including paracrine, gap junction and TNTs-like structures, could mediate the intercellular calcium waves in BV-2 microglial cells.