中文摘要：

瞄准：为了调查 ghrelin 的保护的效果和机制，对 hypoxia/reoxygenation (H/R ) 的 postconditioning 在人的胃的上皮的房间导致了损害。方法：H/R 损害的模型在胃的上皮的房间线(GES-1 ) 被建立人的胃的上皮的房间。房间被划分成七个组：正常控制组(N) ；H/R postconditioning 组；DMSO postconditioning 组(DM ) ；ghrelin postconditioning 组(GH ) ；D-Lys3-GHRP-6 + ghrelin postconditioning 组(D + GH ) ；capsazepine + ghrelin postconditioning 组(C + GH ) ；并且 LY294002 + ghrelin postconditioning 组(L + GH ) 。3-(4,5-dimethylthazol-2-yl )-2,5-diphenyl tetrazolium 溴化物(MTT ) 试金被用来检测 GES-1 房间生存能力。染色的 Hoechst 33258 uorochrome 和流动 cytometry 被进行决定 GES-1 房间的 apoptosis。Spectrophotometry 被执行决定版本喂奶使脱氢(LDH ) 。Bcl-2， Bax， Akt，和肝糖 synthase kinase (GSK )-3 的蛋白质表示被西方的弄污决定。vanilloid 受体子类型 1 的表示(VR1 ) ， Akt 和 GSK-3 被 immunocytochemistry 观察。结果：与 H/R 组相比， GH 组的房间生存能力显著地以一种剂量依赖者方式被增加(55.9%?

英文摘要：

AIM： To investigate the protective effect and mechanisms of ghrelin postconditioning against hypoxia/reoxygenation （H/R）-induced injury in human gastric epithelial cells. METHODS： The model of H/R injury was established in gastric epithelial cell line （GES-1） human gastric epithelial cells. Cells were divided into seven groups： normal control group （N）; H/R postconditioning group; DMSO postconditioning group （DM）; ghrelin postconditioning group （GH）; D-Lys3-GHRP-6 ＋ ghrelin postconditioning group （D ＋ GH）; capsazepine ＋ ghrelin postconditioning group （C ＋ GH）; and LY294002 ＋ ghrelin postconditioning group （L ＋ GH）. 3-（4,5-dimethylthazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） assay was used to detect GES-1 cell viability. Hoechst 33258 fluorochrome staining and flow cytometry were conducted to determine apoptosis of GES-1cells. Spectrophotometry was performed to determine release of lactate dehydrogenate （LDH）. Protein expression of Bcl-2, Bax, Akt, and glycogen synthase kinase （GSK）-3β was determined by western blotting. Expression of vanilloid receptor subtype 1 （VR1）, Akt and GSK-3β was observed by immunocytochemistry. RESULTS： Compared with the H/R group, cell viability of the GH group was significantly increased in a dosedependent manner （55.9% ± 10.0% vs 69.6% ± 9.6%, 71.9% ± 17.4%, and 76.3% ± 13.3%）. Compared with the H/R group, the percentage of apoptotic cells in the GH group significantly decreased （12.38% ± 1.51% vs 6.88% ± 0.87%）. Compared with the GH group, the percentage of apoptotic cells in the D ＋ GH group, C ＋ GH group and L ＋ GH groups significantly increased （11.70% ± 0.88%, 11.93% ± 0.96%, 10.20% ± 1.05% vs 6.88% ± 0.87%）. There were no significant differences in the percentage of apoptotic cells between the H/R and DM groups （12.38% ± 1.51% vs 13.00% ± 1.13%）. There was a significant decrease in LDH release following ghrelin postconditioning compared with the H/R group （561.58