中文摘要：

目的：建立HPLC法测定口服养阴通脑颗粒方后大鼠血清中阿魏酸浓度的方法。方法：健康SD大鼠ig养阴通脑颗粒水溶液后,制备含药大鼠血清。HPLC法测定大鼠含药血清中阿魏酸的含量。采用phenomenex GEMINI C18柱色谱柱,甲醇-3%乙酸（32∶68,v∶v）为流动相;流速1.0 mL.min-1;检测波长323 nm。结果：阿魏酸0.33~10.56μg.mL-1与峰面积线性关系良好（r=0.999 2）,平均回收率为96.41%,RSD 2.36%（n=6）;平均日内精密度RSD2.04%,平均日间精密度RSD5.04%,以信噪比S/N=3时测得的最低检测限为2.6 ng,血浆中的最低检测浓度为0.075μg.mL-1。结论：该方法简便,灵敏度高,稳定性及精密度较好,可用于大鼠血清中阿魏酸的含量测定。

英文摘要：

Objective： To develop an HPLC method for determination of ferulic acid concentrated in the serum of SD rats treated by Yangyin Tongnao Granules（YTG）decoction.Methods：SD rats serum containing Chinese herbal formula by intragastric administrating YTG was prepared.The determination was carried out with HPLC,using phenomenex GEMINI-C_（18） column,methanol,and3% acetic acid（32∶68） was used as mobile phase with a flow rate of 1.0 mL·min~（-1）,and at the detection wave length of 323 nm.Results： The calibration curve showed a good linearity over the range of 0.33～10.56 μg·mL~（-1）（r=（0.999 2）.） and the average recovery for 96.41%,RSD=2.36%（n=6）,RSD=2.04% was less within day and RSD=5.04% was less than 10% day-to-day.and the minimum detection amount was 2.6ng and the minimum detection concentrations in serum was 0.075 μg·mL~（-1）,when the signal-to-noise ratio S/N=3.Conclusion： This method was rapid,sensitive,stable and exact,It was appropriate for the quantitative determination of ferulic acid in the rat serum.