中文摘要：

背景：目前国内外应用类同轴相位衬度技术对生物组织的成像多为多血管成像,很少有对淋巴管的成像研究。另外对淋巴水肿常规影像学检查的分辨率为毫米级,不利于早期诊断,而类同轴相位衬度成像技术可以实现对软组织的微米级成像。目的：利用类同轴相位成像技术实现对兔下肢淋巴水肿模型的微观成像,以观察早期水肿的病变程度。设计、时间及地点：样本于2009-05/06在首都医科大学医学图像实验室制作完成,成像实验于2009-06在上海光源BL13W1线站完成。材料：采用5只新西兰兔制备下肢淋巴水肿模型。方法：利用淋巴结根治术实现实验动物的下肢淋巴水肿,应用类同轴相位衬度CT实现对实验样本的投影成像。对所得投影数据利用滤波反投影法、表面绘制技术进行三维重建。主要观察指标：淋巴管的病变程度,管壁厚度变化。结果：利用同步辐射光相位衬度成像技术得到的下肢淋巴水肿三维衬度图像可以明显的观察到淋巴管道有扩张、弯曲、变形现象发生,并可清晰地观察到淋巴管和静脉血管为促进淋巴回流所支生的细小侧支。结论：利用同步辐射类同轴相衬成像可以实现对淋巴水肿模型的微米级成像,并可观察到样本的早期病变,对临床诊断有一定的意义。

英文摘要：

BACKGROUND： The conventional X-ray imaging has low absorption contrast for lymphoedema, and the resolution is about mm level, which is not conducive to early diagnosis. Micro-imaging of biological soft tissues can be achieved by phase-contras imaging technique. OBJECTIVE： Using synchrotron radiation in-line phase contrast imaging technique to achieve the lymphoedema model imaging and observe the lesions characteristic of lymphatic vessels. DESIGN, TIME AND SETTING： Observational experiment. The lymphoedema model was prepared at the Capital Medical University from May to June in 2009. And the experiment was completed in Shanghai Synchrotron Radiation Facility （SSRF） BL13W1 in June 2009. MATERIALS： Five New Zealand rabbits were prepared for lower extremity lymphoedema models, METHODS： The lower extremity lymph node eradicates operation was performed on rabbits to result in lower extremity lymphoedema. And the synchrotron radiation in-line phase contrast imaging technology was used to collect the projected image of specimens within the 180 o, and to reconstruct three-dimensional image. MAIN OUTCOME MEASURES： Severity of lymphatic vessels and the wall thickness. RESULTS： The lymphatic vessel was swollen, bent and distorted. And the lymphatic vessel generates lateral branches in order to promote lymphatic drainage, which was clearly observed from the image obtained by phase contrast imaging technology. CONCLUSION： The use of synchrotron radiation type coaxial imaging technology can be achieved for lymphedema model for micro-imaging, which has certain significance to the early diagnosis of lymphedema.