中文摘要：

精原干细胞（spermatogonial stem cells，SSCs）具有自我更新和分化的功能，这两种功能的平衡协调不仅能维持其自身数量的稳定，还能满足雄性动物精子生成的需要。近几年，由于细胞培养技术、基因工程技术、生殖细胞移植技术的建立和完善，使SSCs自我更新调控机制的研究取得了许多突破，主要体现在蛋白调控因子和微小RNA分子以及DNA甲基化新作用的发现等方面。该文将着重围绕调控SSCs自我更新的外源性细胞因子和内源性转录因子等蛋白因子进行综述，以期为哺乳动物SSCs的深入研究提供借鉴。

英文摘要：

Spermatogonial stem cells （SSCs） possess both self-renewing division and differentiating division functions, the balance of the two functions not only maintains the stem cell number stable but also meets the demand of the testis to produce millions of sperms each day. Recently, because of the establishment and perfection on techniques of culture in vitro, genetic engineering and germ cell transplantation, the largest break-through in selfrenewal regulation mechanism of SSCs has already been made, including the new discoveries of protein regulatory factors, MicroRNA and the DNA methylation. The present review mainly focuses on the protein regulatory factors such as extrinsical cytokines and specific endogenous transcription factors controlling the SSCs self-renewal, which purposes to provide ideas on the mammalian SSCs.