中文摘要：

摘要昆虫具有高效的先天性免疫系统，血淋巴在免疫过程中发挥重要作用。收集感染家蚕微孢子虫（Nb）后第2、4、6、8天的家蚕幼虫血淋巴，分别以相同发育时期健康家蚕幼虫的血淋巴为对照，进行蛋白质SDS-PAGE电泳分析，寻找家蚕免疫应答Nb侵染的相关蛋白质。结果显示，从感染Nb第6天起，幼虫血淋巴蛋白质组成发生改变，感染第8天的血淋巴样品中出现了3奈明显下调的差异蛋白条带。利用线性离子阱质谱技术（Linear Ion Trap Quadrupole，LTQ）鉴定差异蛋白务带，主要有肌动蛋白3（Actin3）、载脂蛋白（apolipophorin protein）、性别特异储存蛋白2（ sex-specific storage-protein 2 precursor）、储藏蛋白（storage protein）、酚氧化物酶亚基（phenoloxidase subunit）、转铁蛋白（transferrin）和热激相关蛋白70（heat shock cognate protein 70），这7种蛋白质分别在家蚕的能量代谢、先天性免疫以及蛋白质合成、运输、修复等方面扮演重要角色。家蚕感染Nb后血淋巴中的上述7种蛋白质的表达变化，推测可能会影响蚕体自身对Nb的免疫防卫反应能力。

英文摘要：

Insects possess a highly efficient immune system. Insect hemolymph plays an important role in its innate im- mune processes. In order to investigate silkworm （ Bombyx mori） proteins in response to Nosema bombycis （Nb） infec- tion by SDS-PAGE, silkworm hemolymph samples were collected at 2, 4, 6 and 8 days after infection with Nb and hemo- lymph samples from healthy silkworm were used as the control. The results showed that larval hemolymph protein compo- sition on the 6th day post-infection was changed. Especially, there were three obviously down-regulated differential pro-tein bands in hemolymph on the 8th day post-infection. Differential protein bands were identified with linear ion trap quadrupole （LTQ） technique. Seven proteins, namely Actin3, apolipophorin protein, sex-specific stor- age-protein 2 precursor, storage protein, phenoloxidase subunit, transferrin and heat shock cognate protein 70, were indentified. These proteins play important roles in energy metabolism, innate immunity, and in synthesis, transport and repair of proteins. It is suggested that the expression variation of these proteins after Nb infectionmay reduce immune ability of silkworm against Nb infection and lead to a more suitable host environment for reproduction of Nb spores.