研究茄病镰孢菌(Fusarium solani)对铁皮石斛(Dendrobium officinale)的致病性。试验组通过穿刺接种将茄病镰孢菌菌悬液接种于铁皮石斛组培苗及成苗的根部和叶片,植物发病后从病变植株中分离致病菌,并结合形态和分子生物学方法进行鉴定。正常对照组接种无菌水。对照组的铁皮石斛正常生长,试验组的铁皮石斛发生不同程度的病变,病原菌经形态和分子生物学鉴定显示为茄病镰孢菌。茄病镰孢菌对铁皮石斛有致病性。
The pathological research was performed by infecting Dendrobium officinale with Fusarium solani to see the pathogenicity level. The experimental groups were inoculated with F. solani suspension solution on roots and leaves of D. of- ficinale seedling by puncture. When the plants were infected, the pathogens were isolated from the plant and identified by morphological and molecular methods. The control groups were applied with sterile water instead. The results showed that the control groups grew normally, but the experimental groups expressed different levels of lesions. The isolated pathogens were identified as F. solani by both of morphological and molecular methods. F. solani was a virulent pathogen to D. officinale.