中文摘要：

目的探讨重组人促红细胞生成素（rHuEPO）对脑缺血再灌注小鼠海马肿瘤坏死因子α（TNF-α）表达的影响。方法将96只昆明小鼠随机分为：假手术（sham）组、rHuEPO治疗组和缺血再灌注（I/R）组,每组32只。每组依据缺血后再灌注不同时间点再分为6 h、24 h、3 d及7 d 4个小组。应用免疫组化和Western blot检测各组小鼠海马TNF-α蛋白的表达,RT-PCR方法检测TNF-αmRNA表达的变化。结果免疫组化和Western blot检测结果发现,I/R组TNF-α蛋白表达量明显高于sham组和rHuEPO治疗组（P均〈0.05）;RT-PCR结果发现,I/R组TNF-αmRNA表达量明显高于sham组和rHuEPO治疗组（P〈0.05）。结论rHuEPO可通过减少TNF-α的表达参与脑缺血再灌注损伤的神经保护作用。

英文摘要：

Objective To study the expression of tumor necrosis factor-α（TNF-α） in cerebral ischemia-reperfusion mice after recombinant human erythropoietin（rHuEPO） was injected.Methods Ninety six Kunming mice were randomly divided into sham group,ischemia-reperfusion（I/R） group,rHuEPO group at different time points（6 h,24 h,3 d and 7 d） on average.The expression of TNF-α in hippocampus of all groups was examined by immunochemistry and Western blot methods.The expression of TNF-α mRNA was examined by RT-PCR method.Results The positive expression of TNF-α was increased significantly in I/R group than in sham group（P 〈 0.05）,however the expression of TNF-α was lower in rHuEPO group than in I/R group（P 〈 0.05）.The expression of TNF-α mRNA increased significantly in I/R group than in sham group（P 〈 0.05）,however decreased in rHuEPO group.Conclusion rHuEPO has protective effect possibly through down-regulating the expression of TNF-α in cerebral ischemia-reperfusion injury.