中文摘要：

目的：来源于植物β-葡萄糖苷酶活性检测的方法学研究。方法：以对硝基苯基β-D-葡萄糖苷（pNPG）为底物,β-葡萄糖苷酶为催化剂催化底物生成对硝基苯酚,用紫外分光光度法测定对硝基苯酚的含量,根据生成的对硝基苯酚含量计算酶活性,并对此进行方法学研究。结果：对硝基苯酚标准曲线的相关系数为0.999;催化反应的线性相关系数为0.999;反应精密度的相对标准偏差为0.05%;方法重复性的相对标准偏差为2.8%;方法耐用性考察中,酶活性均大于1200 U/g,九个不同方法参数的酶活性相对标准偏差为4.6%;中间精密度实验中,十二份样品酶活性的相对标准偏差为6.6%;稳定性实验中,β-葡萄糖苷酶与底物反应后,放置0小时、16小时、22小时后,测定的酶活性均大于1200 U/g,相对标准偏差为1.1%。结论：此方法线性较好,有良好的方法重复性、精密度、中间精密度、耐用性和溶液稳定性,适用于来源于植物的β-葡萄糖苷酶活性检测。

英文摘要：

Objective： Methodology research for the activity test of β-glycosidase from plants was conducted. Methods： The substrate of 4＇-Nitrophenyl-β-D-glucopyranoside （pNPG） was catalyzed by β-glycosidase to generate 4-nitrophenol. The enzymatic activity of β- glycosidase was determined through assay the concentration of 4-nitrophenol by the mean of ultraviolet spectrophotometry, and the methodology research for this method was fully evaluated. Results： The correlation coefficient of standard curve for 4-nitrophenol was 0.999; The linear correlation coefficient for catalytic reaction was 0.999; The relative standard deviation for precision was 0.05%; The relative standard deviation for method reproducibility was 2.8%; The enzyme activity was more than 1200 U/g and the relative standard deviation was 4.6% for nine different method parameters in the observation of method for durability; The relative standard deviation of enzyme activity for 12 samples was 6.6%, respectively, in the experiment of intermediate precision; For the stability experiment, three time points （0 hour, 16 hour, and 22 hour after the reaction ） were measured. The result shows that the activity of β-glycosidase is above 1200 unit/g after the reaction with the substrate （0 hour）, 16 hours later, as well as 22 hours later, where the RSD is 1.1%. Conclusion： The developed analytical method showed good linearity, repeatability, precision, intermediate precision, stability and durability. It can be applied in the determination of the activity for β-glycosidase from plants origin.