γ-氨基丁酸(GABA)具有降压、降血糖、抗癌等益生功能。玉米低聚肽(CP)作为一种生物活性肽一方面富含产GABA的发酵底物谷氨酸,另一方面可以通过抑制血管紧张素转移酶(ACE)起到降血压的作用。本研究利用酶解法制备玉米低聚肽,并以其为基质经植物乳杆菌NDC75017进行发酵,开发一种富含GABA的乳酸菌饮料。实验利用反向液相色谱法(Re—HPCC)检测发酵液中GABA的含量,利用酶解法制备玉米肽。并对玉米肽的脱苦工艺进行了初探,最后选用β-环糊精包埋苦味肽。得出GABA的产量最高为16mg/100mL,且饮料口感状态优良。产品在4℃贮藏21d后GABA维持在14mg/100mL以上,活菌数(对数值)维持在7.0mL^-1以上。
Gamma-aminobutyric acid (GABA) has various physiological functions, such as hypotensive, hypoglycemic and anticancer activities. Corn oligopeptides (CP) as a bioactive peptides, can not only provide glutamic acid as the material to produce GABA, but also has,the ability to decrease blood pressure by inhibiting angiotensin I-converting enzyme (ACE). This study selected CP as the basis to be fermented by LactobaciUus plantarum NDC75017, and develop a yogurt-like beverage with high nutritive value, which enriched GABA. The concentrations of GABA in the fermentation supematant was determined using reversed-phase high performance liquid chromatography (R.P-HPLC), and hydrolyzed corn gluten meal to CP using alkali protease. Primary research about debittering of CP was also Carried out. Finally, we coated bitter peptides with β -cyclodextrin. The resuks showed the highest content of GABA was reached 16 mg/100 mL. The viable counts of L. plantarum NDC75017 was over 7.0 log CFU/mL after storage for 21 days at 4 ℃ ,with more than 14 mg/100 mL of GABA.