中文摘要：

在有批方法的溶解酵素蛋白质结晶化的进程期间，固体 / 液体的系统的宏观的流动领域被粒子图象 velocimetry (PIV ) 观察。而且，正常生长率(110 ) 脸和本地流动回答在附近作为壁炉蛋白质，水晶被一台长工作距离显微镜获得。试验性的结果证明平均速度，宏观的固体 / 液体的系统的最大的速度和在单个蛋白质水晶附近的本地流动地的速度是变动的。有效界面层厚度 4ft，在接口 C 的集中，和典型速度 V 用 aconvection 散开模型被计算。结果证明在这个实验的溶解酵素水晶的生长被界面的动力学而非体积统治运输，和功能驾驶 ofbuoyancy 的流动大批然而，运输是小的在晶体成长的体积运输的效果有一个趋势随溶解酵素集中的增加增加。计算结果也证明数量级砍力量是大约 10 ~(-21) N，它多不到在溶解酵素分子之间的契约力量。因此， shear 力量导致了驾驶 bybuoyancy 的流动不能把蛋白质分子从水晶的接口移开。

英文摘要：

During the process of lysozyme protein crystallization with batch method, the macroscopic flow field of solid/liquid system was observed by particle image velocimetry （PIV）. Furthermore, a normal growth rate of （110） face and local flow field around a single protein crystal were obtained by a long work distance microscope. The experimental results showed that the average velocity, the maximal velocity of macroscopic solid/liquid system and the velocity of local flow field around single protein crystal were fluctuant. The effective boundary layer thickness δeff, the concentration at the interface Ci and the characteristic velocity Vwere calculated using a convection-diffusion model. The results showed that the growth of lysozyme crystal in this experiment was dominated by interfacial kinetics rather than bulk transport, and the function of buoyancy-driven flow in bulk transport was small, however, the effect of bulk transport in crystal growth had a tendency to increase with the increase of lysozyme concentration. The calculated results also showed that the order of magnitude of shear force was about 10^21 N, which was much less than the bond force between the lysozyme molecules. Therefore the shear force induced by buoyancy-driven flows cannot remove the protein molecules from the interface of crystal.