采用相差显微镜研究了两种杂质蛋白(荧光标记的溶菌酶和商品溶菌酶中的杂质)对四方溶菌酶晶体表面形貌、{110}和{101}表面生长速度以及{110}面台阶生长动力学的影响.杂质会影响晶体表面二维生长岛的形貌,并显著降低晶体{110}和{101}面法向生长速度,杂质会显著降低{110}表面台阶的生长速度,且对(110)方向速度的抑制效果显著强于(001)方向,根据切向台阶速度和过饱和度的关系,计算得到了台阶动力学系数β和台阶的有效表面能及棱边自由能。
The influences of impurity on surface morphology and growth kinetics of tetragonal lysozyme crystals were studied. There was a significant difference in morphology of 2D islands on a {110} face when the impurities existed. Impurities significantly decreased the normal growth rates of both the {110} and {101} face. F-lysozyme decreased the normal growth rate of both the {110} face and {101} face. The values of kinetic coefficient of the steps, surface free energy, and ledge free energy of the step edge were determined.