考察温度对鱼类低氧耐受生化反应的影响,以白鲢(Hypophthalmichthysmolitrix)幼鱼为对象;分别在15、30℃条件下驯化2周;随后将实验鱼置于密闭呼吸室中通过鱼体自发耗氧进行低氧处理,并以不经历急性低氧处理的实验鱼为对照组;分别测定实验鱼的血液和白肌中乳酸、白肌和肝脏中的糖原、葡萄糖含量。实验结果显示,经急性低氧处理后高、低温组血乳酸含量均显著上升(p〈0.05),而低氧处理后15℃下肌糖原和30℃下肝葡萄糖含量却显著降低(p〈0.05);随着温度的升高(不论低氧处理与否)肌乳酸含量均显著降低(p〈0.05),而温度的升高并未改变实验鱼的肌葡萄糖以及肝葡萄糖的含量;统计检验结果表明温度与低氧处理间不存在交互作用。研究表明,白鲢幼鱼在面临温度变化和低氧胁迫时鱼体内代谢底物和无氧代谢产物水平均产生相应的变化,这种变化对实验鱼适应不同温度环境及低氧胁迫有着重要的意义。
In order to investigate the effects of temperature on biochemical reactions of hypoxia tolerance in fish, we select juvenile silver carp (Hypophthalmichthys molitrix) as the experimental animal. All the fish were divided into two groups (15 and 30 ℃ ) for temperature acclimation for two weeks then the half fish of each temperature group were put into the closed respirometer cham- bers for experiencing a treatment of acute hypoxia through spontaneous oxygen consumption individually, the other half fish without the treatment of acute hypoxia of each temperature group as the control. The blood lactate, white muscle lactate, white muscle gly- cogen, liver glycogen and liver glucose content of each group were measured. The blood lactate content of each temperature group increased significantly after acute hypoxia (9〈0.05) while both the muscle glycogen content at 15 ℃ and liver glucose content at 30 ℃ decreased significantly after hypoxia (p 〈0.05). The muscle lactate content of both hypoxia and normoxia groups decreased sig- nificantly with the increase of the temperature (p 〈0.05). The glucose content of both muscle and liver showed no change with temperature. Furthermore, there was no interaction effect between temperature and hypoxia. The study showed that when undergo- ing the change of temperature and hypoxic situation, both the metabolic substrates and anaerobic metabolic products changed corre- spondingly in juvenile silver carp, to handle the thermal and hypoxia stresses.