中文摘要：

目的：探讨磷脂酶Cε1（PLCE1）抑制肺腺癌A549细胞凋亡的作用机制。方法：选用人肺腺癌细胞株A549作为研究对象。采用real-time PCR和Western blotting法分别检测PLCE1抑制剂U-73122处理前、后肺腺癌细胞株A549中PLCE1和p53 mRNA和蛋白水平的表达；流式细胞术检测细胞凋亡。结果：肺腺癌细胞株A549高表达PLCE1，低表达p53；抑制PLCE1表达后A549细胞中p53表达上调，细胞凋亡明显增加。结论：PLCE1通过抑制肺腺癌A549细胞株中p53的表达，从而抑制A549细胞凋亡。

英文摘要：

AIM：To investigate the role of phospholipase C epsilon 1 （ PLCE1 ） in modulating the apoptotic mechanism in lung adenocarcinoma A 549 cells.METHODS：PLCE1 inhibitor U-73122 was used to suppress the expres-sion of PLCE1.The expression of PLCE1 and p53 in A549 cells was evaluated by quantitative real-time PCR and Western blotting.Apoptosis was assessed by flow cytometry .RESULTS：A549 cells expressed high level of PLCE1 and low level of p53.Inhibition of PLCE1 markedly increased the expression of p 53, and increased the apoptosis of A 549 cells.CON-CLUSION：PLCE1 suppresses apoptosis of A549 cells via inhibiting the expression of p53.