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中文摘要：
以外消旋体2-苯基丙酸为原料，探讨不同醇对2-苯基丙酸酯化反应的影响，确定异丙醇为最佳醇底物．通过微水相优化拆分制备S-2-苯基丙酸的反应条件，确定了青霉素酰化酶拆分制备S-2-苯基丙酸的最佳工艺参数．实验结果表明，95％四氢呋喃水溶液中，酶浓度1．5mg／mL，底物浓度0．3g／mL，30℃，pH7为最佳酶拆分反应条件，S-2-苯基丙酸的收率为94．5％，ee值为97．0％．
英文摘要：
A new bio-enzymatic catalysis procedure of producing enantiomeric S-2-phenylpropionic acid was investigated. The effect of different alcohols on the esterification of 2-phenylpropionic acid was performed and the optimal alcohol was isopropanol. S-2-phenylpropionic acid was obtained by hydrolysis of 2-phenylpropionic ester catalyzed by penicillin G acylase （PGA） in the micro-aqueous phase. In the 95% （φ） tetrahydrofuran solvent system, the optimal conditions were obtained as follows： enzyme PGA at 1.5 mg/mL, 2-phenylpropionic ester 0.3 g/mL, 30 ℃, and pH 7. The maximum yield and ee value of S-2-phenylpropionic acid were 94.5% and above 97.0%, respectively.
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