中文摘要：

目的研究十二烷基硫酸钠（SDS-NaOH）溶液对尿液中的革兰阳性和革兰阴性细菌的鉴别能力。方法用SDS-NaOH溶液处理79份尿液标本5min，以UF-1000i尿液分析仪检测处理前后的尿液中的细菌计数水平，并计算细菌比值（BR）。以受试者工作特征（ROC）陷线分析BR对革兰阳性和革兰阴性细菌的鉴别能力。结果革兰阴性细菌的BR较革兰阳性细菌降低（P=0．01）。BR鉴别革兰阳性和革兰阴性细菌的曲线下面积为0．70（95％可信区间：0．57～0．82）。当BR取值为0．05时，鉴别革兰阳性细菌的敏感性为0．83（95％可信区间：0．63～0．95），特异性为0．46（95％可信区间：0．32～0．59）。结论SDS-NaOH处理有助于鉴别尿液中革兰阳性和革兰阴性细菌，该法鉴别快速且易于自动化。

英文摘要：

Objective To explore the efficiency of SDS-NaOH solution for discriminating Gram-positive and Gram- negative bacteria in urine. Methods Seventy-nine urine samples were treated with SDS-NaOH solution for 5 minutes. The bacterium counts were determined by UF-1000i urine analyzer before and after treatment, and the bacteria ratio （BR） was calculated. Receiver operation characteristics （ROC） curve analysis was used to assess the discrimination efficiency of BR. Results BR in Gram-negative bacteria contained urine was significantly lower than that in Gram-positive one （P=0.01）. The area under curve （AUC） of BR for discriminating Gram-positive and Gram-negative bacteria was 0.70 （95 % confidence interval： 0. 57-0. 82）. At a cut-off value of 0.05, the discrimination sensitivity and specificity of BR for Gram-positive bacteria were 0.83 （95% confidence interval：0.63-0, 95） and 0. 46 （95% confidence interval：0.32-0.59）, respectively. Conclusion Treatment with SDS-NaOH solution can help to discriminate Gram-positive and Gram-negative bacteria in urine, and the method is rapid and automatic.