中文摘要：

目的：采用高效液相色谱法建立桑椹药材的HPLC指纹图谱,为其品质控制提供可靠依据。方法：采用HPLC-UV分析桑椹的指纹图谱。色谱柱：Agilent XDB C18柱（4.6 mm×250 mm,5μm）,流动相：乙腈-0.3%磷酸溶液梯度洗脱,流速：0.5 mL/min,柱温：35℃,检测波长：290 nm。测定的10批桑椹指纹图谱,应用相似度分析对桑椹药材进行研究。结果：在高效液相指纹图谱中,确定了12个共有峰,经过相似度分析,10批药材中7批相似度较好,其中3批相似度偏低。结论：桑椹药材指纹图谱特征性及专属性强,可为药材的质量控制提供依据。

英文摘要：

Objective：To establish HPLC fingerprint of Morus alba from different habitats by HPLC and provide basis for its quality control.Methods： HPLC analysis was performed on an Agilent XDB C18 Column（4.6 mm×250 mm,5 μm）,gradient eluted composed of acetonitrile and 0.3 % phosphate acid.The column temperature was set at 35 ℃ and the flow rate was 0.5 mL/min.The detective wavelength was 290 nm.The HPLC fingerprint for 10 batches of Morus alba was studied on their similarity.Results：There were twelve common peaks in the fingerprint.The similarity of 7 batches was above 0.9 and the other batches had low similarity.Conclusion：The HPLC fingerprint can be used for quality control of Morus alba with high characteristics and specificity.