中文摘要：

在体外模拟人体消化环境,测定了不同脱乙酰度和粘均分子量的壳聚糖对脂肪、胆固醇和胆盐的结合能力,探讨了壳聚糖物化性质对结合能力的影响。结果表明,壳聚糖结合脂肪能力受脱乙酰度和粘均分子量共同影响而呈现规律性变化,表现为随着这两个指标的增高而相应增强,而结合胆固醇能力则没有变化,但随着其粒径的增大,结合能力下降。壳聚糖结合胆盐能力受粘均相对分子质量影响较大,分子量高,则结合能力强,不同脱乙酰度的壳聚糖结合能力相差不大。说明壳聚糖物化性质对其结合能力有影响,影响大小取决于结合物和壳聚糖之间的作用机制。

英文摘要：

The physico-chemical properties of chitosans have the correlationship with their functional properties. It has proved that chitosan has the potent hypocholesterolemic activity in vivo. In this paper, the effects of degree of deacetylation （DD） and viscosity-average molecular weight （Mv） of chitosan samples on their fat-binding, cholesterol-binding and bile salts-binding capacities were studied in vitro. Nine chitosan samples with different DD and Mv were prepared by chemical methods. The binding capacities of fat, cholesterol and bile salts were determined in the conditions simulating human gastrointestinal tract. The optimal additions of samples for binding assays are 120 mg fat-b~ding assay and 40 mg bile salts-binding, respectively. The fat-binding capacity ranges from 11 -37.4 g fat/g chitosan higher than that of cellulose significantly and it increases with the increase of DD and Mv. The cholesterol-binding capacity does not show the regularity variation with the changes of the DD and Mv, but is affected by the diameter of the granule. The bile salts-binding capacity is 1.75-7.75 mg/g chitosan. The viscosity-average molecular weight affects the binding capacity greatly. Chitosan sample with highest molecular weight shows the best bile salts- binding capacity. The degree of deacetylation seems to have no effect on the bile salts-binding capacity. These results indicated that the physicochemical properties of chitosan affect its binding capacities and hypocholesterolemic and hypolipidemic activities.