中文摘要：

研究了周期性机械拉伸对角膜成纤维细胞外基质中金属蛋白酶-2（Matrixmetalloprotei-nase-2，MMP-2）、组织金属蛋白酶抑制剂-2（Tissue inhibitors of metalloproteinase-2，TIMP2）及转化生长因子-β1（Transforminggrowthfactorp1，TGF-β1）表达的影响。对正常兔角膜成纤维细胞进行幅度分别为5％、10％及150o的周期性机械拉伸，并分别于拉伸后6h、24h取细胞培养液，采用ELISA方法检测MMP-2、TIMPl2及TGF-β1的含量。当拉伸幅度为5％时，6h、24h后，MMP-2、TIMP-2及TGF-β1的含量与静态对照组相比均无统计学差异；当拉伸幅度为10％时，6h后MMP-2、TIMP2及TGF-β1的含量与静态对照组相比无统计学差异，24h后MMP-2的含量显著升高（p〈O．05）；当拉伸幅度为15％时，6h后MMP-2、TIMP-2及TGF-β1的含量与静态对照组相比仍无统计学差异，拉伸24h后MMP-2、TGF-β1含量显著升高，TIMP-2含量显著降低（声〈0．05）。结果表明，在体外以一定的幅度周期性拉伸角膜成纤维细胞一定时间后，细胞分泌的MMP-2、TIMP-2及TGF-β1发生显著改变，三者相互作用，共同调节角膜成纤维细胞的生物学行为。

英文摘要：

To investigate and determine the effect of cyclic mechanical stretch on the expres- sion of Matrix metalloproteinase-2 （MMP-2）, Tissue inhibitors of metalloproteinase-2 （TIMP-2） and Transforming growth factor β1 （TGF-β1）in extracellular matrix remodeled by corneal fibro blasts,corneal fibroblasts were isolated from normal rabbits and subjected to a cyclic stretch regi- men of 5%, 10% and 15% elongation. The concentration of MMP-2, TIMP-2 and TGF-β1 was assayed on aliquots of culture medium by ELISA method after stretched for 6 h and 24 h separate ly. Our results show that; When the amplitude of stretch was 5%, there was no significant difference in MMP-2, TIMP-2 and TGF-β1 among groups at 6 h or 24 h;When the amplitude of stretch was 10%, there was also no significant difference in MMP-2, TIMP-2 and TGF-β1 among groups at 6 h,but level of MMP-2 was significantly（p〈0.05） elevated at 24 h;When the ampli- tude of stretch was 15%, there was also no significant difference in MMP-2, TIMP-2 and TGF-βI among groups at 6 h, but level of MMP-2, TGF-β1 was significantly（p〈0.05） elevated and TIMP-2 decreased at 24 h. It indicates that MMP-2, TIMP-2 and TGF-βI interacted together to mediate the corneal biology behavior when the cornea was subjected to proper mechanical stimula tion.