欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
为了增强胆固醇酯转移蛋白（CETP）羧基末端的26肽B细胞抗原表位的免疫原性，以大肠杆菌L-门冬酰胺酶Ⅱ（AnsB）为载体，通过引入2个强的辅助T细胞表位，构建了融合基因AnsB-TTP-PADRE-CETPC，并在大肠杆菌BL-21（DE3）表达，经过硫酸铵沉淀、DEAE 52纤维素阴离子交换层析、分子筛凝胶过滤层析，最终得到单一电泳条带纯度的目的蛋白。纯化的融合多肽疫苗免疫新西兰大白兔可诱导产生高水平持续存在的抗CETP抗体，从而抑制了CETP酶活力，显著抑制了兔主动脉斑粥样硬化斑的形成和发展。该融合蛋白有希望作为抗动脉粥样硬化疫苗进一步研究开发。
英文摘要：
In order to increase the carboxy-terminal B cell epitope immungenicity of human CETP, a fusion protein AnsB-TTP-PADRE-CETPC was successfully constructed, expressed and purified to immunize New Zealand white rabbits for inducing high titers of anti-CETP antibodies to inhibit the formation and development of aortic atheromatous patch. The protein was expressed as soluble protein in Escherichia coli and purified with anion exchange column and by gel filtration chromatography after ammonium sulfate precipitation. After immunizing rabbits with the purified protein, high titer anti-CETP antibodies were induced and it lasted more than nineteen weeks in vivo; patch areas in aorta of rabbits were significantly reduced compared with control rabbits. Therefore, the fusion protein AnsB-TTP-PADRE - CETPC might be further developed into a vaccine against atherosclerosis.
同期刊论文项目
同项目期刊论文
HSP65 serves as an immunogenic carrier for a diabetogenic peptide P277 inducing anti-inflammatory im
A novel virus-like particle based on hepatitis B core antigen and substrate-binding domain of bacter
Strong humoral response elicited by a DNA vaccine targeting gastrin-releasing peptide with optimized
Immunotherapy of Autoimmune Diabetes by Nasal Administration of Tandem Glutamic Acid Decarboxylase 6
Study on preparation and unique properties of a novel insulin analogue with N-terminal Arg-4, Pro-3,
Improved efficacy of DNA vaccination against prostate carcinoma by boosting with recombinant protein
Nasal immunization with heat shock protein 65 attenuates atherosclerosis and reduces serum lipids in
Specific Antibodies Elicited by a Novel DNA Vaccine Targeting Gastrin-Releasing Peptide Inhibit Muri
Construction, expression, purification and immunology effect of an anti-atherosclerosis chimeric enz
A novel vaccine targeting gastrin-releasing peptide: efficient inhibition of breast cancer growth in
A Th1-recognized peptide P277, when tandemly repeated, enhances a Th2 immune response toward effecti
Study on preparation and activity of a novel recombinant human parathyroid hormone(1-34) analog with
Intranasal immunization with chitosan/pCETP nanoparticles inhibits atherosclerosis in a rabbit model
Low molecular weight chitosan as carrier for gene delivery: in vivo efficiency and intramucosal tran
期刊信息
位置：