中文摘要：

目的：探讨大鼠心肌缺血再灌注不同时间点基质金属蛋白酶（MMP）-1，2，9的表达及其意义。方法：建立大鼠在体心肌缺血再灌注损伤模型，采用免疫组化法检测心肌组织中MMP-1．2．9的表达，以四通道电生理仪检测心功能，比色法测定血浆肌酸激酶（CK）、乳酸脱氢酶（LDH）和髓过氧化物酶（MPO）活性。结果：与假手术组相比缺血再灌注组心功能明显降低，CK、LDH和MPO活性显著增高，呈明显的时间依赖性。于再灌注2h达到峰值（P〈0.01）。缺血再灌注后大鼠心肌中MMP-1的表达水平明显高于假手术组和缺血组（P〈0．05），以再灌注1h最为明显（P〈0．01），且与心功能的改变呈负相关（r=-0．503～-0．748，P均〈0.01）；MMP2没有表达；MMP-9的表达于再灌注1h开始增强，再灌注2h达到高峰（P〈0．01），与心功能呈负相关（r=-0．732～-0．855。P均〈0.01）。结论：MMPs可能参与心肌缺血再灌注损伤过程。

英文摘要：

Objective： To investigate the changes and significance of MMP-1, 2, 9 in myocardium of rats during myocardial ischemia-reperfusion （I/R）. Methods： The models of I/R were successfully established in anaesthetized rats. Immunohistochemical SABC method .and.computerized image analysis were used to observe alterations of MMP- 1, 2, 9 protein in myocardium of rats. The function of left ventricle was measured by instrument of collecting physiological signals. The activities of CK, LDH , MPO were assayed by colorimetry method. Results： There were obviously deterioration in the left ventricular function and the activities of CK, LDH and MPO increased （P〈0.01 all） after ischemia-reperfusion; those changes were time-dependent. The expression of MMP- 1, 9 were significantly enhanced after ischemia-reperfusion （P〈0.05） and reached peak at the first h, second h of reperfusion in myocardium of rats respectively （P〈0.01 all）. The expression of MMP-1, 9 were negatively correlated with the changes of heart function during myocardial ischemia-reperfusion （r=- 0. 503 - - 0. 855, P〈0. 01 all）. The MMP-2 could not be detected by immunohistochemistry with the anti-MMP-2 antibody. Conclusion.. MMPs may play a key roles in myocardial ischemia-reperfusion injury.