中文摘要：

目的通过克隆凝血因子C同源物基因（Coagulation factor Chomology，COCH）全长cDNA，为COCH编码蛋白cochlin的功能研究打下基础。方法从听力正常人新鲜外周静脉血中提取总RNA，应用一步法RT～PCR试剂盒进行COCH反转录，转录产物进行浓缩、酶切、连接。结果反转录COCHcDNA全长与标准基因序列比较，结果完全相符，得到完整的COCHcDNA全长序列。结论本研究成功克隆了COCHcDNA全长序列，为COCH编码蛋白cochlin的功能研究打下了良好的基础。

英文摘要：

Objective To obtain full--length cDNA of human coagulation factor C homology （COCH） gene. Methods Total RNAs were extracted from the peripheral venous blood. One step RT--PCR kit was used to carry out reverse transcription. The transcript was condensed, digeseted by Hind Ⅲ restriction enzyme and ligated by DNA ligation kit. Results Comparing with standard gene order from gene bank, the acquired full--length eDNA sequence was coincident eompletely. Conclusion In our studies, the full--length eDNA of human coagulation factor C homology was cloned successfully, it will facilitate our future studies on functions of coehlin protein.