缓解重金属污染对植物伤害的方法是目前研究的热点之一。铜(Cu)是植物生长发育所必需的微量营养元素。过量的Cu则对植物的生长发育有抑制作用。钾(K)是植物生长所必需的营养元素,施用钾肥(K2SO4)能明显增加植物的抗逆性。为了探讨外源K对Cu胁迫下延胡索植株生理生化响应的调节作用,为延胡索高效经济,优质高产的栽培模式提供理论与实践指导,以小叶型延胡索(Cf)和大叶型延胡索(C)为研究材料,采用土培的方法,研究外源K对Cu胁迫下2个延胡索叶型植株生长、抗氧化酶系统和Cu积累等的影响。结果表明:各浓度Cu胁迫下外源K的最适缓解浓度均为200 mg/kg。200 mg/kg K能明显缓解Cu胁迫对2种叶型(C和Cf)植株生长的抑制作用,增加植株的耐Cu性;尽管存在一定的叶型差异,但外源K能使2种叶型叶片中的超氧化物歧化酶(SOD)、过氧化物酶(POD)及过氧化氢酶(CAT)的活性降低,降低了叶片中的可溶性糖含量和丙二醛(MDA)含量,从而缓解Cu对延胡索植株的毒害。另外,外源K影响Cu对延胡索植株地上部分和地下部分Cu的积累,其影响程度与延胡索叶型有关,其中能显著降低大叶型延胡索植株地下部分Cu含量(p〈0.05)。机制较复杂。
Alleviation method of heavy metal pollution damage to the plant has become one of the hot points in the world. Copper (Cu) is an essential trace nutrient element for plant growth and development, however its excessive accumulating in soil is becoming a severe threat to crop production as well as human health. The presence of excessive amount of Cu may cause toxicity to plants, resulting in stunted growth, strong reduction in biomass production, damage of the photosynthetic apparatus, reduction in chlorophyll content and disturbance of mineral nutrition and carbohydrate metabolism. Potassium (K) is an essential nutrient element for plant growth and development, and application of potassium fertilizer (KzSO4) can significantly increase the resistance of plants to heavy metal stress. To understand the regulative effect of K on the physiological and biochemical responses of Corydalis yanhusuo plants under Cu stress,two C. yanhusuo cultivars, C ( Corydalis yanhusuo ) and Cf( Corydalis yanhusuo flowers ) grown in soil were used to investigate the effects of exogenous K on the growth of plants, activities of antioxidant enzymes (SOD,POD and CAT) and Cu accumulation in two C. yanhusuo under 400 mg/kg Cu stress. The results showed the optimal alleviation concentration for K was 200 mg/kg under all levels of Cu stress in terms of plant height and biomass of plants. The addition of 200 mg/kg K significantly alleviated the inhibitory effect of Cu stress on the growth of C. yanhusuo plants. K also decreased the activities of superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT), the contents of soluble sugar and malondialdehyde (MDA) in the leaves of C. yanhusuo plants under Cu stress, despite of certain leave type difference, suggesting that K could alleviate oxidative damage of C. Yanhusuo plants caused by Cu. In addition, exogenous K supply affected Cu accumulation of the shoots and roots of C. Yanhusuo plants under Cu stress. The effects had significant leaf type difference