中文摘要：

目的：研究用β-葡萄糖苷酶酶解淫羊藿苷制备宝藿苷Ⅰ的工艺。方法：采用β-葡萄糖苷酶酶解淫羊藿苷制备宝藿苷Ⅰ，以转化率为指标，通过单因素试验考察温度、pH值、酶与底物质量比、底物浓度和反应时间对转化率的影响。结果：酶解反应的优化条件为温度50℃、pH=5．0的枸橼酸-枸橼酸三钠缓冲液、酶与底物质量比1：2、底物浓度1mg·mL^-1、反应时间90min。结论：β-葡萄糖苷酶酶解淫羊藿苷制备宝藿苷Ⅰ，转化率高，工艺简单、可靠，反应条件温和。

英文摘要：

OBJECTIVE： To study the preparation technology of baohuoside Ⅰ by enzymolysis of icariin with β-glucosidase. METHODS： Taking bioconversion rate as index,β-glucosidase was used for enzymolysis of icariin and the effects of temperature, pH value, the ratio of enzyme to substrate, substrate concentration and reaction time on conversion rate of baohuoside Ⅰ were investigated by single factor test. RESULTS： The optimum reaction conditions were determined as follows： 50 ℃, citric acid-citric acid sodium acetate buffer （pH 5.0）, the ratio of enzyme to substrate 1：2, substrate concentration 1 mg.mL-1 and reaction time 90 min. CONCLUSION： It is simple and reliable to prepare baohuoside Ⅰ byby the enzymolysis of icariin with β-glucosidase; the conversion rate is high and the technology is moderate.