采用过氧化物酶同工酶(POD)和酯酶同工酶(EST)对菊属8个种植物的27份材料(不同地理居群或品种)的遗传多样性进行了分析.结果表明:(1)27份材料共获得12个同工酶位点,以及13种POD条带和15种EST条带;(2)27份材料用UPGMA聚类为两组,第Ⅰ组为野生菊、药用菊,第Ⅱ组为观赏菊,前者品种较为原始,后者品种较为进化;(3)野菊12个不同居群的平均观察杂合度(Ho)为0.166 7~0.750 0,其中南京野菊遗传变异最大,河南野菊最小;(4)菊属8个种的遗传一致度为0.461 6~0.860 5,平均遗传距离为0.451 7,其中栽培菊与野菊的亲缘关系最近(遗传一致度为0.860 5),野菊可能是栽培菊的一个主要起源种.
The genetic diversity of 27 materials in 8 species of Dendrantherna was studied based on the polymorphisms of POD and EST isozymes. The results showed that (1) a total of 13 POD bands and 15 EST bands from 12 isozyme loci were obtained; (2) The UPGMA phenogram of 27 materials showed that the first group were all the wild ones and the medicinal chrysanthemum which were primordial, and the second group were the ornamental ones which were evolutive; (3) The mean observed heterozygosity (Ho) among 12 populations of D. indicurn was between 0. 166 7 and 0. 750 0. The genetic variation of Nanjingyeju(D. indicum) was the highest,and that of He' nanyeju was the lowest in the populations; (4)Nei's genetic identity of 8 species in Dendranthema was between 0. 461 6 and 0. 860 5,and the average genetic distance was 0.451 7. Nei's genetic identity between Dendrantherna × grandiflorurn and D. indicura was 0. 860 5, which showed that the two species had the nearest relationship and D. indicum was probably one of the original species of Dendrantherna X grandi florurn.