中文摘要：

选择青岛地区喜食的阿拉斯加狭鳕、蓝点马鲛、大黄鱼、真鲷、鲑、大菱鲆、鲤等7种鱼为研究对象,利用鱼类过敏患者血清,通过SDS-PAGE及免疫印迹方法对每种鱼的过敏原蛋白进行鉴定。利用兔抗鲅鱼小清蛋白多克隆抗体,通过间接ELISA和抑制性ELISA等对7种鱼的小清蛋白之间的交叉反应进行探讨。结果表明,青岛地区居民鱼类过敏原主要为48～57、33～41、28及17 ku的蛋白,和传统公认的小清蛋白为主要过敏原的共识不同。而针对小清蛋白的交叉反应研究也表明,不同鱼类蛋白之间存在强烈的交叉反应。因此,虽然鱼类过敏原可能不同,但如果对以上7种鱼中的一种鱼过敏,最好不要食用其他任何一种鱼。

英文摘要：

Qingdao is a coastal city of China where the problem of fish allergy is increasingly prominent.There have been no data providing a comprehensive picture of the major fish allergens to local people so far.Based on the above situation,the aim of this research was to study on the cross-reactivity of different fish allergens,to provide primary basis of safe eating guide for local fish allergic individuals.To determine the allergens of 7 commonly edible fishes：Alaska pollock（Theragra chatcogramma）,red snapper（Pagrosomus major）,salmon（Salmo salar）,spanish mackerel（Scomberomrus niphonius）,turbot（Scophthatmus maximus）,large yellow croaker（Larimichthys crocea） and carp（Cyprinus carpio）,SDS-PAGE was applied to observe the protein components of fish extracts.Sera from 18 fish allergic patients were used in Western-blot.Cross-reactivity was investigated by IgG Western-blot,IgG ELISA and ELISA inhibition used rabbit anti-spanish mackerel parvalbumin polyclonal antibody.Results showed that the major fish allergens were 48-57,33-41 ku,28 ku and 17 ku proteins to Qingdao residents,which was distinct from the common consensus that parvalbumin was the major allergen in fish.Meanwhile,the study on the cross-reactivity of fish parvalbumin also indicated there was a quite strong cross-reactivity between different species of fish proteins.Therefore,despite the allergens might be different from each other,it was suggested that the subjects not eat other 6 fishes if allergic to one of them.