以伊犁金山葡萄庄园的红提葡萄为原料,通过比较常规葡萄和有机葡萄不同器官表面附生菌群的差异,旨在揭示常规和有机两种不同栽培模式对葡萄附生菌群的影响。葡萄附生菌的分离、筛选方法为平板培养法,微生物的计数方法为最大或然数法。结果显示:葡萄根部附生菌的群落总数最大(105CFU/mm2),茎次之,果实最小(约102CFU/mm2)。在果实上,有机葡萄附生真菌种类多于常规葡萄,而附生细菌种类与之相反。除葡萄叶没有显著差异外,有机葡萄各器官(根、茎、果实)附生菌菌落总数均大于常规葡萄。初步表明,有机栽培可以更好地保持生态环境中微生物群落的生物多样性。
Both organic and conventional red globe grape were sampled from Jinshan vineyard in Yili. The effect of different farming models of conventional and organic farming models on the epiphyte distribution was studied preliminarily in current paper by comparing the epiphytic biodiversity in grape organs. The agar-plate culture method was used for the microbial isolation, and the most-probable-number (MPN) was used for the enumeration of epiphyte population. The result was as follows: the largest number of epiphytes located on the root (up to 10^5 CFU/mm^2), and the stem took second place, and the smallest on fruit (about 10^2 CFU/mm^2). Besides, the amount of epiphytic fungi species of the organic grape was slightly higher than that of conventional grape; on the contrary, the amount of epiphytic bacteria species of the organic grape was lower. The number of epiphytic microorganisms of organic grape was more than that of conventional grape on all organs except leaf. The organic farming was shown to be more effective to keep the biodiversity of microbial communities in ecological environment.