中文摘要：

目的探讨胃癌组织中PTEN、vascular endothelial growthfactor（VEGF）基因表达及其与肿瘤侵袭转移的关系。方法用RT-PCR和免疫组化方法检测胃癌、淋巴结转移组织中PTEN、VEGF mRNA和蛋白表达;用CD34检测肿瘤细胞微血管数。结果PTEN和VEGF mRNA表达阳性率在正常胃黏膜为76.5%与0.0%、胃癌组织为30.9%与69.1%、淋巴结转移组织23.6%与74.5%;PTEN和VEGF蛋白阳性率在正常胃黏膜为76.5%与0.0%、胃癌组织27.9%与82.4%、淋巴结转移组织16.3%与91.0%;胃癌组织中新生血管呈浸润生长,以淋巴结转移组织中明显。胃癌组织PTEN mRNA和蛋白低于正常胃黏膜（P〈0.01）,VEGF高于正常胃黏膜（P〈0.01）,PTEN与VEGF表达负相关（P〈0.05）,VEGF表达与新生血管形成正相关（P〈0.05）。结论PTEN基因失活和VEGF的过表达与新生血管形成相关,可能是通过调节包括VEGF在内的血管生成因子而在血管形成中起作用。

英文摘要：

Objective To investigate the expression of PTEN and VEGF genes and their correlation with tumor metastasis in gastric cancer. Methods RT-PCR and immunohistochemistry were carried out to detect the expression of PTEN and VEGF in gastric cancer tissue and metastatic lymph nodes. The micro-angiogenesis of gastric tumor tissue was analyzed by CD34 immunohistochemical staining. Results The positive rates of PTEN and VEGF mRNA expression were 76.5% and 0.0% in normal mucosa, 30.9 % and 69.1% in gastric cancer tissue, and 23.6 % and 74.5 % in metastatic lymph nodes, respectively, The positive rates of PTEN and VEGF proteins expression were 76.5% and 0.0% in normal mucosa. 27.9% and 82.4% in gastric cancer tissue, and 16.3% and 91.0% in metastatic lymph nodes, respectively. The angiogenesis in gastric tumor tissue showed a infiltrative and invasive growth, especially in the metastatic lymph nodes. The PTEN mRNA and protein expression were less than that in normal gastric mucosa （P 〈 0.01 ）. The VEGF mRNA and protein expression in the gastric cancer tissue was higher than that in normal gastric mucosa （P 〈 0.01 ）. The correlation between PTEN and VEGF mRNA expression was negative and the angiogenesis was positively related to VEGF expression （ P 〈 0.05 ）. Conclusion PTEN gene inactivation and VEGF overexpression are related with angiogenesis. PTEN may play a role in angiogenesis through regulating angiogenin including VEFG.