中文摘要：

目的 检测核因子E2 p45相关因子2（Nuclear factor-erythroid 2 p45-related factor 2,NRF2）与多药耐药相关蛋白2（multidrug resistance-associated protein 2,MRP2）在原发性胆囊癌组织中的表达,探讨其在胆囊癌发生、发展中的作用.方法 采用免疫组化SP法检测59例原发性胆囊癌组织中NRF2和MRP2的蛋白表达水平.结果 原发胆囊癌组织中NRF2和MRP2表达的强阳性率分别为76.3%和74.6%.随着转移程度、Nevin分级以及病理分级的增加,NRF2和MRP2的强阳性表达率增加,且差异具有统计学意义（P＜0.05）;两者的强阳性表达与年龄、性别等临床特征无相关性;NRF2和MRP2表达之间具有相关性（r=0.589,P＜0.05）.结论 NRF2和MRP2在原发性胆囊癌组织存在高表达且具有相关性,在胆囊癌的发生、发展中发挥作用.

英文摘要：

Objective To detect the expressions of Nuclear factor-erythroid 2 p45-related factor 2 （NRF2） and multidrug resistance-associated protein 2 （MRP2）, and investigate their significance in primary gallbladder carcinoma. Methods Immunohistochemistry SP assay and image analysis were used to detect the expressions of NRF2 and MRP2 protein in 59 patients with primary gallbladder carcinoma. Results A highly positive expression rates of NRF2 and MRP2 were found （76.3% and 74. 6%, respectively） in primary gallbladder carcinoma. The expressions of NRF2 and MRP2 had a significantly correlation with metastases, Nevin staging, and differentiation （P〈0.05）, but there was no statistical association with sex and age. The expression of NRF2 had a positive correlation with MRP2 （r=0. 589,P〈0.05）. Conclusion Both NRF2 and MRP2 were overexpressed in primary gallbladder carcinoma and they may play a role in the development of primary gallbladder carcinoma.