中文摘要：

目的：探讨健脾解毒方在幽门螺杆菌（Helicobacter pylori，H．pylori）长期感染诱发C57BL／6小鼠胃癌过程中对微血管密度（microvessel density，MVD）和血管新生相关基因的调控作用，为其治疗H．pylori感染相关胃病提供实验依据。方法：采用胃．pylori标准株SSlig的方法建立 H．pylori感染C57BL／6小鼠诱发胃癌动物模型，分为对照组、模型组、健脾解毒方低剂量组和健脾解毒方高剂量组。72周时，免疫组化法检测各组小鼠胃黏膜MVD的变化；实时荧光定量PCR（RFQ—PCR）法和免疫组化法检测各组小鼠胃黏膜血管内皮生长因子（vascular endothelial growthfactor，VEGF）、血管生成素-2（angiopoietin．2，Ang-2）及其受体Tie-2 mRNA和蛋白的表达。结果：对照组、模型组、健脾解毒方低、高剂量组胃黏膜MVD分别为（2．50±1．54），（18．56±2．62），（14．61±3．60），（7．39±1．75）个／视野。模型组MVD较对照组明显增高，P〈0．01；健脾解毒方低、高剂量组MVD较模型组显著降低，P〈0．01。模型组VEGF，Ang-2，Tie-2mRNA表达较对照组都明显增加，P〈0．01；健脾解毒方低、高剂量组均可显著降低三者的表达，并成一定的剂量依赖效应。结论：H．pylori诱发C57BL／6小鼠胃癌过程中，可增加胃黏膜MVD，促进VEGF，Ang-2，Tie-2的表达，在H．pylori诱导的胃癌发生中起到促进作用；健脾解毒方可减少MVD，抑制VEGF，Ang-2，Tie-2的表达，这可能是其防治胃癌的重要机制之一。

英文摘要：

Objective： To investigate the regulatory effects of Jianpi Jiedu Recipe on microvessel density （MVD） and angiogenesis-related genes in the course of Helicobaeter pylori（H, pylori） long-term infection induced gastric adenocarcinoma of C57BL/6 mice, and provide experimental basis for Jianpi Jiedu Recioe treatment of the H. pylori-related gastropathy. Method： C57BL/6 mouse gastric cancer model induced by H. pylori infected was established by gavage with H. pylori standard strain SS1, and mice were divided into 4 groups： Control （Cont） group-model（Mod） group and low-dose（LD） and high-dose（HD） Jianpi Jiedu Reeioe group. After 72 weeks, MVD in gastric mucosa of each group was detected with Evision immunohistoehemical method. Vascular endothelial growth factor（VEGF）, angiopoietin-2 （Ang-2） and Ang-2 type 2 receptor（Tie-2） mRNA and protein expression were evaluated by real-time fluorogenic quantitative polymerase chain reaction （ RFQ-PCR ） and immunohistochemical method. Result： Pathological examination demonstrated that at the 72 th week, the mice gastric mucosa MVD were 2.50 ± 1.54, 18.56 ±2.62, 14.61 ±3.60 and 7.39 ± 1.75 in Cont, Mod, LD and HD group respectively. The MVD in Mod group was obviously increased than that in Cont group, P 〈 0.01; The MVD were significantly decreased in LD and HD Jianpi Jiedu Recioe group, compared with Mod group, P 〈 0.05,P 〈 0.01. FRQ-PCR suggested that the expression of VEGF, Ang-2, Tie-2 mRNA and protein in Mod group were obviously increased than those in Cont group, P 〈 0.01 ; However both the LD and I-ID Jianpi Jiedu Recioe could significantly reduce their expression in a dose-dependent manner. Conclusion： H. pylori could increase MVD of gastric mucosa, and induce VEGF, Ang-2 and Tie-2 expression in the course of H. pylori infection-induced gastric adenocarcinoma of C57BL/6 mice, which might play an important role in the incidence of H. pylori-induced gastric cancer. Jianpi Jiedu Recipe could decrease MVD, and inhibit VEG